Vivarium caters to disease model development in rat, mice, rabbits and zebra fish. Laboratory Animal Models Zebra fish Models Experimental Protocol set up, customize study design, monitoring, reporting, data and statistical analysis for Preclinical Studies Animal species: mice, rats, guinea pigs, hamsters, and rabbits and invertebrates like Zebra fish and you have the option to choose based on your end points Route of administration: oral, subcutaneous, intradermal, intravenous, intraperitoneal, mixed with food and water, topical, retro-orbital, compounds added in fish tank, intramuscular Measurements in biological fluids and organs: Plasma, urine, faeces, Blood and serum clinical chemistry, clinical observation, Dissection of organs and tissues, biochemical markers ( enzymes like GSH, SOD, catalase) , immunological markers, inflammatory markers( cytokine and interleukin) & IHC staining and gene markers histopathological observation Technique: Imaging, Microscopy, special staining, absorbance, fluorescence, luminescence, blotting, RTPCR, gel electrophoresis Lab Animal Research-Pharmacological efficacy animal Models and Toxicity: Animal models is conducted to mimic the biological process in disease condition and executed in rats, mice, rabbits, zebra fish and others are likely in their anatomy, physiology and reaction to various stimuli to human physiology and behavior. Cardiometabolic disorders : • Anticoagulant studies, MI/arrhythmias, Angina, Heart failure, Hypertension Diabetic and Metabolic Models: • Acute Dyslipidemia Model: corn oil / intralipid induced, streptozotocin/alloxan induced diabetes • Chronic Model: High-Fat diet-induced obesity mouse model, zebra fish models Obesity model: • Diet-induced obesity in rat, mice, zebra fish, Fructose-induced hypertriglyceridemia, Hypercholesterolemia model Respiratory diseases • Asthma, COPD, Anaphylaxis /allergy CNS Disorders: • Neurological disorders Memory/cognition, Anxiety, Depression, Epilepsy, ADHD, Brain & Spine Injury Models, Parkinson’s Disease, Cognition / Motivation, Schizophrenia & Mania, Alzheimer’s Disease-Scopolamine Treated Mice and zebra fish models, Catalepsy, Neuroinflammation, Analgesic models. Inflammation: • TNBS, oxazolone, DSS induced colitis Arthritis: • Collagen /Pristane /Adjuvant Induced Arthritis, osteoarthritis • Carrageenan-induced chronic inflammatory arthritis GI: • Ulcers, GI transit time, GI emptying Oncology: • Tumor models – cell line/chemical induced Ovarian, Breast, Lung, Prostate, Brain, Gastric, Pancreatic, Colon, Epidermoid carcinoma, Melanoma, Leukaemia, etc. Dermatology/Skin disorder: • Dermal /Wound Healing / Burn Models/ Inflammation Ophthalmology: • Diabetic retinopathy (DR) model-rodent and zebra fish , Choroidal neovascularization (CNV) model-Mouse, Rat, zebra fish , eye disease model- Glaucoma ocular model in Mouse, rat, rabbit and zebra fish Biotechnology Immunization in rats, mice, rabbits for production of polyclonal antibodies. ELISA, Western blot, RTPCR, absorbance and fluorescence based assays are executed, live cell imaging with inverted microscope and florescence microscope Cell proliferation and cytotoxicity Toxicity • Exploratory toxicity studies, pharmacokinetic study, Toxicokinetic profiling and PK/PD studies • Acute, subacute, chronic toxicity studies in rats, mice, rabbits and zebra fish as per OECD/ICH/Regulatory guidelines, Oral, Intravenous, intraperitoneal injection, Histopathological studies and interpretation by veterinary pathologist Bioavailability • Pharmacokinetics / Toxico-kinetics/ Metabolite Profiling/Excretion/Organ distribution
We provide in-vivo pharmacokinetic study in rats, mice, rabbits and zebra fish, bio-analysis supporting all phases of drug discovery and development.Non-GLP rapid discovery phase pharmacokinetics and bio-analysis for discovery support. Our capabilities include proprietary method development, validation, transfer, and high throughput sample analysis using LCMS, HPLC, fluorescence, absorbance techniques. Also take up organ tissue distribution study and in-vitro ADME studies. analysis like ELISA, chromatography, blotting. absorbance and fluorescence measurements using multimode reader
We support in herbal extraction, phytochemical analysis, fermentation techniques for isolation of compounds, metabolites and production of enzymes, cell line based specialized cellular assays, fluorescent imaging, staining with DAPI, acridine orange, Propidium iodide etc. Read out using multimode reading for fluorescence, absorbance, luminescence, DNA/RNA quantitation, blotting, imaging techniques. Gene and protein expression studies are conducted. Assays like wound healing, cytotoxicity, apoptosis, anticancer, organ toxicity are carried out with cell culture facility
Discovery and development programs covering various therapeutic areas including metabolic diseases, oncology, CNS diseases, parkinson, alzheimer, anxiety, locomotor, inflammation, pain, dermatology and infectious diseases which require determination of activity using screening assays, animal studies in rats, mice and rabbits and also alternative model in zebra fish. Techniques invitro assays,in-vivo models, ELISA, absorbance, fluorescence, luminescence, PCR, imaging, spectrophotometric and colorimetric readouts
We offer a full range of in-vivo pharmacokinetic studies for the test compound and identification of metabolites in rodents (rats, mice,rabbits, hamster, guinea pigs, hamster) and zebra fish. we adopt different route of administration like oral, intravenous, intramuscular, subcutaneous, nasal, retro orbital, vitreal etc in rats, mice, rabbits, zebra fish as appropriate. We collect biological matrices like blood, plasma, serum, tissues and organs, faeces,urine for bioanalysis of the test compounds The studies in large animals (Beagle dogs and cats, sheep) done with collaborator.
Determination of activity using in-vitro methods like cells, cell line, enzyme,and in-vivo methods using rats, mice, rabbits and zebra fish disease models to test compounds for their activity. It determines potency and effective dose or effective concentration EC50 or ED50 of compounds so that we can rank order the test compounds based on their value in the biological system. Techniques to measure the enzyme or cell activity adopts absorbance, fluorescence, luminescence, ELISA, imaging to get their mechanism of action and activity. Antioxidant, anti-inflammatory, antimicrobial, anticancer, cytotoxicity, hepatoprotective, neuroprotective, cytotoxicity etc are determined for the test molecules. We use cell free as well cell based assays and use molecular mechanism to understand the role of gene and protein pathways (by separation of DNA RNA protein from tissues to study MOA). Disease induction in done in rats, mice rabbits and zebra fish to know the molecule effectiveness in the preclinical evaluation during drug development stage
The in vivo preclinical animal facility at Aaranya Biosciences offers a full range of non-GLP safety pharmacology and toxicology evaluations in rats, mice, rabbits and zebra fish. Toxicity study based on acute, subacute and chronic are conducted and route of administration of drugs like oral, nasal, intravenous, intramuscular, ocular, subcutaneous are done based customer needs. Pharmacokinetic and toxicokinetic studies are done in biological samples includes plasma, blood, serum, organs. Also carry out protein based expression profile and gene regulation studies in specific tissues. Measure biomarkers using ELISA, spectrophotometer, chromatographic methods. We also deal in diseases relating to cardiovascular, CNS, pulmonary, renal, gastrointestinal, and autonomic nervous system safety pharmacology assessments and measure organ specific toxicity using cell lines or ex-vivo studies. Panel of biomarkers as specified by clients are carried
We can help you with hands-on creation of liquid types or dry types of drug formulations, provide you with access to centers of expertise that provide unique new technologies, assist you with patent and literature reviews with recommendations for development, offer expertise in state-of-the art formulations, and help your team with drug formulations technology presentations.
We have time tested business models for each pharma and bio industry clients' specific needs in the pharma and life sciences industries. Our flexible and strategic models combine our location advantage and proven competencies. With us, you can expect complete CRO/CMO services.