PLATFORM : In-vitro Cell-based assays and assay development for compound screening.

TARGETS : Gene and protein levels.

TECHNOLOGIES: ELISA, PCR, qPCR, FISH, Immunohistochemistry, LC/MS/MS, Transfection, Western blot, etc

PRELIMINARY ANALYSIS

• Cell proliferation
• Cytotoxicity test
• Cell apoptosis

LIVER HEALTH

Characterization of markers involving in liver injury, fibrogenesis or fibrolysis Human HSC LX-2, rat HSC T6, primary rodent HSC and hepatocyte systems

• Non Alcoholic Steatohepatosis (NASH) assay
• Liver Cirrhosis models
• Cystinosis model
• Hepatoprotection against toxins (tBHP, Ethanol, organ-pesticides etc)
• Enzyme induction studies using HepG2

INFLAMMATION

Cytokine/chemokine production assay for inflammatory response/inhibition assessment Stimuli: LPS, PMA, PHA, Con A CD3/CD28, etc.

• T cell and B cell proliferation in response to specific antigens or mitogens
• Trans-well chemotaxis assay with eosinophils, neutrophils, monocytes, B cells, T cells, etc.
• Inflammation related cell signaling pathway study

Gene expression profile using PCR and Western-blot The assays are performed with primary cells (such as PBMCs, eosinophils, neutrophils, monocytes, B cells, T cells, etc.) and cell lines (THP1, Jurkat, Raw264.7, etc.) using a variety of technologies.

IN-VITRO INFLAMMATORY MODELS WITH MONOCYTES:

• IL-1beta inhibition
• IL-6 inhibition
• Nitric oxide release assay
• TNF-alpha inhibition assay
• PGE2 inhibition
• LTB4 inhibition
• TXB2 inhibition
• Histamine inhibition

IN-VITRO IMMUNO-MODULATORY MODELS WITH BLOOD CELLS/ SPLENOCYTES

• IL-2
• IL-3
• IL-4
• IL-5
• IL-6
• IL-10
• IL-12
• PGE2
• IFN

GENOTOXICITY PANELS

• AMES
• Micronucleus
• Chromosomal aberration
• Mouse lymphoma assay

ONCOLOGY PANELS

COSMETOLOGY PANELS

• Melanin inhibition
• Collagen,
• Elastin secretion.

DIABETICS AND OBESITY MODEL

• Adipolysis and adipogenesis using 3T3-L1, C3H10T1/2 cell lines
• Glucose uptake studies

BONE HEALTH

• ALP release
• Calcium deposition using C3H10T1/2 cell lines

ENDOCRINE DISRUPTOR SCREENING

• Steroidogenesis
• Receptor Binding studies
• Transcription and activation studies