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ASSAY LIST


PLATFORM
:

In-vitro Cell-based assays and assay development for compound screening.

TARGETS :

Gene and protein levels.

TECHNOLOGIES :

ELISA, PCR, qPCR, FISH, Immunohistochemistry, LC/MS/MS, Transfection, Western blot, etc



PRELIMINARY ANALYSIS
  • Cell proliferation

  • Cytotoxicity test

  • Cell apoptosis

LIVER HEALTH

Characterization of markers involving in liver injury, fibrogenesis or fibrolysis

  Human HSC LX-2, rat HSC T6, primary rodent HSC and hepatocyte systems


  • Non Alcoholic Steatohepatosis (NASH) assay

  • Liver Cirrhosis models

  • Cystinosis model

  • Hepatoprotection against toxins (tBHP, Ethanol, organ-pesticides etc)

  • Enzyme induction studies using HepG2

INFLAMMATION

Cytokine/chemokine production assay for inflammatory response/inhibition assessment
  • Stimuli: LPS, PMA, PHA, Con A CD3/CD28, etc.

  • T cell and B cell proliferation in response to specific antigens or mitogens

  • Trans-well chemotaxis assay with eosinophils, neutrophils, monocytes, B cells, T cells, etc.

  • Inflammation related cell signaling pathway study

Gene expression profile using PCR and Western-blot

  The assays are performed with primary cells (such as PBMCs, eosinophils, neutrophils, monocytes, B cells, T cells, etc.) and cell lines (THP1, Jurkat,
   Raw264.7, etc.) using a variety of technologies.



IN-VITRO INFLAMMATORY MODELS WITH MONOCYTES:
  • IL-1beta inhibition

  • IL-6 inhibition

  • Nitric oxide release assay

  • TNF-alpha inhibition assay

  • PGE2 inhibition

  • LTB4 inhibition

  • TXB2 inhibition

  • Histamine inhibition

IN-VITRO IMMUNO-MODULATORY MODELS WITH BLOOD CELLS/ SPLENOCYTES

  • IL-2

  • IL-3

  • IL-4

  • IL-5

  • IL-6

  • IL-10

  • IL-12

  • PGE2

  • IFN

GENOTOXICITY PANELS

  • AMES

  • Micronucleus

  • Chromosomal aberration

  • Mouse lymphoma assay


ONCOLOGY PANELS

COSMETOLOGY PANELS
  • Melanin inhibition

  • Collagen,

  • Elastin secretion.

DIABETICS AND OBESITY MODEL

  • Adipolysis and adipogenesis using 3T3-L1, C3H10T1/2 cell lines

  • Glucose uptake studies


BONE HEALTH

  • ALP release

  • Calcium deposition using C3H10T1/2 cell lines


ENDOCRINE DISRUPTOR SCREENING

  • Steroidogenesis

  • Receptor Binding studies

  • Transcription and activation studies

 
  • Computational Chemistry
  • Medicinal Chemistry
  • Molecular Pharmacology
  • DMPK
  • Preformulation
  • Toxicity and Safety Pharmacology
  • Bio-analysis
  • Consulting
  • Disease Models
  • Program Management
  • Integrated Discovery Services
  • Platform Technologies
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