Assay List

PLATFORM: In-vitro Cell-based assays and assay development for compound screening.
TARGETS : Gene and protein levels.
TECHNOLOGIES : ELISA, PCR, qPCR, FISH, Immunohistochemistry, LC/MS/MS, Transfection, Western blot, etc

INFLAMMATION

Cytokine/chemokine production assay for inflammatory response/inhibition assessment

Stimuli: LPS, PMA, PHA, Con A CD3/CD28, etc.

T cell and B cell proliferation in response to specific antigens or mitogens

Trans-well chemotaxis assay with eosinophils, neutrophils, monocytes, B cells, T cells, etc.

Inflammation related cell signaling pathway study

Gene expression profile using PCR and Western-blot

The assays are performed with primary cells (such as PBMCs, eosinophils, neutrophils, monocytes, B cells, T cells, etc.) and cell lines (THP1, Jurkat,
Raw264.7, etc.) using a variety of technologies.

LIVER HEALTH

Characterization of markers involving in liver injury, fibrogenesis or fibrolysis

Human HSC LX-2, rat HSC T6, primary rodent HSC and hepatocyte systems

Non Alcoholic Steatohepatosis (NASH) assay

Liver Cirrhosis models

Cystinosis model

Hepatoprotection against toxins (tBHP, Ethanol, organ-pesticides etc)

Enzyme induction studies using HepG2

IN-VITRO INFLAMMATORY MODELS WITH MONOCYTES:

IL-1beta inhibition

IL-6 inhibition

Nitric oxide release assay

TNF-alpha inhibition assay

PGE2 inhibition

LTB4 inhibition

TXB2 inhibition

Histamine inhibition

IN-VITRO IMMUNO-MODULATORY MODELS WITH BLOOD CELLS/ SPLENOCYTES

IL-2

IL-3

IL-4

IL-5

IL-6

IL-10

IL-12

PGE2

IFN

GENOTOXICITY PANELS

AMES

Micronucleus

Chromosomal aberration

Mouse lymphoma assay

COSMETOLOGY PANELS

Melanin inhibition

Collagen,

Elastin secretion.

DIABETICS AND OBESITY MODEL

Adipolysis and adipogenesis using 3T3-L1, C3H10T1/2 cell lines

Glucose uptake studies

ENDOCRINE DISRUPTOR SCREENING

Steroidogenesis

Receptor Binding studies

Transcription and activation studies